Essay --

Jeffrey Chhim Professor Garth Hall Results Both studies utilized microdialysis probes on rats to obtain dialysates, these were further analyzed through liquid chromatography (LC) and/or mass spectrometry (MS) for the presence of tryptamine derivatives (5-hydroxytryptamine (5-HT or serotonin) and melatonin (study 1), Dimethyltryptamine (DMT) and other molecules (study 2)) in the pineal gland. In the first study, the surgical implantation of microdialysis probe operation was performed on 142 rats with 10% of which deceased during or shortly following surgery before any data can be attained. Of the rats that endured the surgical implantation, another 10% were considered not meet for the study due to the incorrect position of the probes. The second study did not include information about how many rats were utilized and if any had perished during the experimentation process. LC (Study 1) Source [2] Figure 1: Daily summaries of 5-HT (red) and melatonin (yellow) secretion in four categories of rats. In each, the first night represents 24 hours after the probe placement, and each data point represents the extent of recuperation throughout a 10-min interval. Class I (panel A), class II (panel B), class III (panel C), and class IV (panel D), and one representative rat is shown for each class. Horizontal axes signify time of day, and the vertical axes signify the quantity of secretion for every injection. The darken regions signify 01:00–11:00 hours in each panel. Microdialysates of rats were organized into four unique classes based on stable recovery (Panels A-D) refer to Figure 1, each peak height represents the 5-HT secretion during early night as well as the normal daytime secretion with little or no day-to-day variation. Class I (... ...0732/ 161.10735 amu), IAA (176.07061/176.07057 amu), HIAA (192.06552/ 192.06511 amu), TRP (205.09715/205.09711 amu) and HNATA (219.11280/219.11296 amu) identified. Analysis of samples for DMT by precise mass gave 189.13860 amu associated with the calculated exact mass of 189.13866 amu obtained for a DMT reference. DMT was not found in all samples like that of melatonin and HTA. Sources 1. Barker S A, Borjiginm J, Lomnicka I, Straussman R. (2013). Biomedical Chromatography. â€Å"LC/MS/MS analysis of the endogenous dimethyltryptamine hallucinogens, their precursors, and major metabolites in rat pineal gland microdialysate.† DOI 10.1002. 2. Sun X, Liu T, Deng J, Borjigin J. Long-term in vivo pineal microdialysis. Journal Of Pineal Research. [online]. September 2003;35:118-124. Available from: Academic Search Premier, Ipswich, MA. Accessed February 25, 2014.